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Saturday, November 28, 2020

Antibody Titration Anti-A, Anti-B ,Anti-D

 



PRINCIPLE AND APPLICATIONS:  

Antibody titration is a determination of the concentration of a specific antibody in the patient's serum or to determine the strength of antigen expression on different red cell samples. 

If the concentration of the specific antibody is being determined, the cells must contain the known antigen and the procedure should be performed under the optimal conditions for that antibody. 

The usual applications of titration studies are:

  1. estimating the antibody activity of an alloimmunized pregnant female.
  2. attempting to determine if there is any specificity to an autoantibody
  3. characterizing antibodies that may be high titer, low avidity antibodies

SAMPLE

Either plasma and serum can be titrated.  In the case of a pregnant female, frozen serum or plasma from previous months should be run along with the fresh specimen to determine if there is a rise in titer.

REAGENTS, EQUIPMENT, AND SUPPLIES:

  • Reagent cells known to have the antigen that will be reacting with the titered antibody.  If titering for anti-A or anti-B levels, A1 or B Cells would be used.  If titering for anti-D levels, O cells that are homozygous for D should be used.
  • 12 x 75 mm tubes
  • test tube rack
  • marking pen
  • dispo pipettes
  • physiologic saline
  • serofuge
  • lighted agglutination viewer

PROCEDURE

  1. Label 10 tubes according to the serial dilution: 1, 2, 4, 8, 16, 32, 64, 128, 256, 512 and the patient identification.  The first tube will be undiluted serum.  Tube 2 will be a 1/2 dilution, 4 will be a 1/4 dilution. 
  2. Add 0.3 ml of saline to tubes 2 through 512.  No saline in tube 1
  3. Add 0.3 ml of serum to both tubes 1 and 2.
  4. Use a clean pipette to mix the 1/2 dilution several times and then transfer 0.3 ml to tube 4.
  5. Use a clean pipette to mix the 1/4 dilution several times and then transfer 0.3 ml to tube 8.
  6. Continue the process for all dilutions (512).  Remove 0.3 ml from tube 10 (512) and reserve in a clean tube if the titration needs to be continued.
  7. Label a new set of 10 tubes with the appropriate dilutions.
  8. Using a separate pipettes for each dilution, transfer 2 drops of each tube to the appropriate tubes.
  9. Add one drops of specific red blood cells.
  10. Mix well and test by the appropriate technique for the specific antibody.
  11. Examine test results macroscopically, grade and record the reactions

NOTES AND PRECAUTIONS

  • If titrating anti-A, anti-B, or anti-A,B, the serologic technique is performed by the same method as ABO Typing
  • If titrating Rh, Kell, Duffy, or Kidd antibodies, the serologic technique includes a 37oC followed by antiglobulin testing.
  • If testing a pregnant female, each month serum should be compared to the previous month.
  • Prozone phenomenon may occur so the first tubes may have a weaker reaction than the more diluted serum.  AABB recommends reading the most dilute tubes first and then shake out the other tubes.
  • Careful pipetting is essential.
  • Cells with known antigens may have different reactivity and therefore the serum from each month must use the same cells
  • Measurement is more accurate at larger dilution, therefore the larger dilution should be made before smaller volumes are used to test with the red cells antigen.

INTERPRETATION

  • Observe the highest dilution that produces 1+ macroscopic agglutination
  • The titer is reported as the reciprocal of the dilution level:  32 not 1/32
  • A rise in titer would need to be at least 2 dilution increase between the current specimen and the previous month.
  • For identification of high-titer, low-avidity antibodies would generally have a titer of 64 or greater.

Saturday, November 7, 2020

The National Commission for Allied and Healthcare Professions Bill, 2020 was introduced in Rajya Sabha

 

                                     Ministry: 
Health and Family Welfare







  • The National Commission for Allied and Healthcare Professions Bill, 2020 was introduced in Rajya Sabha by the Minister of Health and Family Welfare, Dr. Harsh Vardhan, on September 15, 2020.  The Bill seeks to regulate and standardise the education and practice of allied and healthcare professionals.  Key features of the Bill include:






  • Allied health professional: The Bill defines ‘allied health professional’ as an associate, technician, or technologist trained to support the diagnosis and treatment of any illness, disease, injury, or impairment.  Such a professional should have obtained a diploma or degree under this Bill.  The duration of the degree /diploma should be at least 2,000 hours (over a period of two to four years).
     






  • Healthcare professional: A ‘healthcare professional’ includes a scientist, therapist, or any other professional who studies, advises, researches, supervises, or provides preventive, curative, rehabilitative, therapeutic, or promotional health services.  Such a professional should have obtained a degree under this Bill.  The duration of the degree should be at least 3,600 hours (over a period of three to six years).





  • Allied and healthcare professions: The Bill specifies certain categories of allied and healthcare professions as recognised categories.  These are mentioned in the Schedule to the Bill and include life science professionals, trauma and burn care professionals, surgical and anaesthesia related technology professionals, physiotherapists, and nutrition science professionals.  The central government may amend this Schedule after consultation with the National Commission for Allied and Healthcare Profession.
     




  • National Commission for Allied and Healthcare Professions: The Bill sets up the National Commission for Allied and Healthcare Professions.  The Commission will consist of: (i) the Chairperson, (ii) Vice-Chairperson, (iii) five members (at the level of Joint Secretary) representing various Departments/ Ministries of the central government, (iv) one representative from the Directorate General of Health Services, (v) three Deputy Directors or Medical Superintendents appointed on a rotational basis from amongst medical institutions including the AIIMS, Delhi and AIIPMR, Mumbai, and (vi) 12 part-time members representing State Councils, among others.


  • Important links:- https://www.aiimsexams.org/
  • https://www.aiims.edu/en.html
  • https://aiimsbhubaneswar.nic.in/
  • https://mohfw.gov.in/

  • Functions of the Commission: The Commission will perform the following functions with regard to Allied and Healthcare professionals: (i) framing policies and standards for regulating education and practice, (ii) creating and maintaining an online Central Register of all registered professionals, (iii) providing basic standards of education, courses, curriculum, staff qualifications, examination, training, maximum fee payable for various categories, and (iv) providing for a uniform entrance and exit examination, among others.
     
  • Professional Councils: The Commission will constitute a Professional Council for every recognised category of allied and healthcare professions.  The Professional Council will consist of a president and four to 24 members, representing each profession in the recognised category.  The Commission may delegate any of its functions to this Council.
     
  • State Councils: Within six months from the passage of the Bill, state governments will constitute State Allied and Healthcare Councils.  The State Councils will consist of: (i) the Chairperson (at least 25 years of experience in the field of allied and healthcare science), (ii) one member representing medical sciences in the state government, (iii) two members representing state medical colleges, (iv) two members representing charitable institutions, and (v) two members from each of the recognised categories of allied and healthcare professions, nominated by the state government, among others.  The State Councils will: (i) enforce professional conduct and code of ethics to be observed by allied healthcare professionals, (ii) maintain respective State Registers, (iii) inspect allied and healthcare institutions, and (iv) ensure uniform entry and exit examinations.
     
  • Establishment of institutions: Prior permission of the State Council will be required to: (i) establish a new institution, or (ii) open new courses, increase the admission capacity, or admit a new batch of students to existing institutions.  If such permission is not sought, then any qualification granted to a student from such an institution will not be recognised under the Bill. 
     
  • Offences and penalties: No person is allowed to practice as a qualified allied and healthcare practitioner other than those enrolled in a State Register or the National Register.  Any person who contravenes this provision will be punished with a fine of Rs 50,000.

Friday, November 6, 2020

Allied and Healthcare Professions Bill 2018

Allied and Healthcare Professions Bill, 2018: A much needed regulation but raises questions on implementation


The allied health professionals in India are not covered by medical or nursing councils. Lab technicians, X-ray technicians, ICU technicians or people who are not covered by any other council and are currently unregulated.

Allied and Healthcare Professions Bill, 2018: A much needed regulation but raises questions on implementation

A big announcement, with perhaps far reaching implications in the Indian healthcare sector, was made on Thursday. The Union Cabinet chaired by Prime Minister Narendra Modi approved the Allied and Healthcare Professions Bill, 2018 for regulation and standardisation of education and services by allied and healthcare professionals.
The bill provides for setting up of an Allied and Healthcare Council of India and corresponding State Allied and Healthcare Councils, which will play the role of a standard-setter and facilitator for professions of Allied and Healthcare. A much needed measure many would say.

The allied health professionals in India are not covered by medical or nursing councils. Think: lab technicians, X-ray technicians, ICU technicians or people who are not covered by any other council and are currently unregulated.
There are over 100 such categories across every branch of healthcare - in eye care for instance: optometrists, in ear problem: audiologists, for diabetes care: diabetologists - covering large number of people across the country.
You would want such critically important professionals to be regulated and adhere to high quality. Since, much of this is still wanting, the plan for new councils for them is arguably much needed. But then this bill has been in the works for over five years now, what is clearly apparent is that this is at best a good starting point.
The bigger challenge lies in effectively implementing it. As every institution teaching these professionals will need to be accredited and those coming under regulation, will fight tooth and nail and given the history of healthcare regulation in India, there could be scope for manipulation.
Also, while the act may lay down strict regulations, what happens when the supply of professionals is less than the demand? How will stipulation on higher qualifications help if supply of talent is not matched? Regulation, after all, works best when the regulators as well as those to be regulated understand the need to be disciplined. Are the regulators always well intentioned?
Ask some private healthcare providers and those within the government in turn point to the various training shops that tend to duck the strict standards and regulations.
After all, this act should not be good only on paper and well drafted piece of regulation but be one that is effectively implemented. And the reason people are sceptical is because there is so much money involved that there is always the danger of abuse. How can that be minimised?
State government and medical community must all be on board and young people joining training courses to be such professionals must see the need for standards - laying down the curriculum, syllabus and laying down the period of training.
How many hospitals today publicly declare their rates and are transparent about their pricing? Also, what if the training provided is not matching and is shoddy?
Every provider, be it a pathology lab that is keen on quality or a diabetics chair that wants to retain its reputation, will need to continue what they do today, which is to largely train their own people.
It all boils down again to history of healthcare regulation in India. It certainly has room for improvement and therefore what is being done to ensure that the new councils will not go down the same path and instead chart out a new course? Also, what will be done to ensure that this will not be used by the big and powerful healthcare providers for eliminating smaller players under the garb of enforcing quality?
What be the view on ensuring high quality delivery in tier II and tier III cities and towns, where even doctors are in short supply, not to talk of high quality allied healthcare professionals? How all these questions get addressed, still needs to be seen.